Cry1 and GPA1 signaling genetically interact in hook opening and anthocyanin synthesis in Arabidopsis

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While studying blue light-independent effects of cryptochrome 1 [cry1] photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein alpha subunit [GPA1] null mutant gpa1. Both cry1 a...

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Detalles Bibliográficos
Otros Autores: Fox, Ana Romina, Soto, Gabriela Cynthia, Jones, Alan M., Casal, Jorge José, Muschietti, Jorge Prometeo, Mazzella, María Agustina
Formato: Artículo
Lenguaje:Inglés
Materias:
ARABIDOPSIS THALIANA
CRY1
GPA1
HETEROTRIMERIC G-PROTEINS
SIGNAL TRANSDUCTION
ANTHOCYANIN
ARABIDOPSIS PROTEIN
CRY1 PROTEIN, ARABIDOPSIS
CRYPTOCHROME
GPA1 PROTEIN, ARABIDOPSIS
GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA SUBUNIT
MESSENGER RNA
PLANT RNA
SUCROSE
ARABIDOPSIS
BIOLOGICAL MODEL
BIOSYNTHESIS
DRUG EFFECT
GENE EXPRESSION REGULATION
GENETICS
LIGHT
METABOLISM
MUTATION
PHENOTYPE
PHYSIOLOGY
PLANT GROWTH
PROTEIN BINDING
PROTEIN PROCESSING
RADIATION EXPOSURE
SEEDLING
ANTHOCYANINS
ARABIDOPSIS PROTEINS
CRYPTOCHROMES
GENE EXPRESSION REGULATION, PLANT
GTP-BINDING PROTEIN ALPHA SUBUNITS
HYPOCOTYL
MODELS, BIOLOGICAL
PROTEIN PROCESSING, POST-TRANSLATIONAL
RNA, MESSENGER
RNA, PLANT
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2012Fox.pdf
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Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
Descripción
Sumario:While studying blue light-independent effects of cryptochrome 1 [cry1] photoreceptor, we observed premature opening of the hook in cry1 mutants grown in complete darkness, a phenotype that resembles the one described for the heterotrimeric G-protein alpha subunit [GPA1] null mutant gpa1. Both cry1 and gpa1 also showed reduced accumulation of anthocyanin under blue light. These convergent gpa1 and cry1 phenotypes required the presence of sucrose in the growth media and were not additive in the cry1gpa1 double mutant, suggesting context-dependent signaling convergence between cry1 and GPA1 signaling pathways. Both, gpa1 and cry1 mutants showed reduced GTP-binding activity. The cry1 mutant showed wild-type levels of GPA1 mRNA or GPA1 protein. However, an anti-transducin antibody [AS/7] typically used for plant G alpha proteins, recognized a 54 kDa band in the wild type but not in gpa1 and cry1 mutants. We propose a model where cry1-mediated post-translational modification of GPA1 alters its GTP-binding activity.
ISSN:0167-4412

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