Activation with ionomycin followed by dehydroleucodine and cytochalasin b for the production of parthenogenetic and cloned bovine embryos

In this work, Dehydroleucodine [DhL] was evaluated as a chemical activator of bovine oocytes and somatic cell nuclear transfer [SCNT] reconstituted embryos. Oocytes were activated with 5 uM Ionomycin [Io] and exposed for 3h to 1 or 5 uM DhL alone [Io-Dhl1 or Io-DhL5] or combined with Cytochalasin B...

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Otros Autores: Canel, Natalia Gabriela, Bevacqua, Romina Jimena, Fernández Martín, Rafael, Salamone, Daniel Felipe
Formato: Artículo
Lenguaje:Inglés
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COW
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2010Canel.pdf
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