Molecular identification of the secondary endosymbiont Hamiltonella defensa in the rose - grain aphid Metopolophium dirhodum

This is the first report of the association between the rose-grain aphid Metopolophium dirhodum, a potentially important cereal pest and the facultative symbiont Hamiltonella defensa. The infection with this gamma-proteobacterium was determined by PCR in laboratory-reared and field-collected specime...

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Detalles Bibliográficos
Otros Autores: Telesnicki, Marta Cecilia, Ghersa, Claudio Marco, Martínez Ghersa, María Alejandra, Arneodo, Joel Demián
Formato: Artículo
Lenguaje:Inglés
Materias:
APHIDIDAE
FACULTATIVE SYMBIONT
GAMMA-PROTEOBACTERIA
PCR
SEQUENCING
INTERNAL TRANSCRIBED SPACER
RNA 16S
RNA 23S
APHID
BACTERIUM IDENTIFICATION
GAMMAPROTEOBACTERIA
GENE SEQUENCE
GEOGRAPHIC DISTRIBUTION
HAMILTONELLA DEFENSA
HUMAN
INFECTION RATE
METOPOLOPHIUM DIRHODUM
NUCLEOTIDE SEQUENCE
PHYLOGENETIC TREE
PHYLOGENY
POLYMERASE CHAIN REACTION
ANIMALS
APHIDS
PROTEOBACTERIA
SEQUENCE ANALYSIS, RNA
SYMBIOSIS
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2012Telesnicki.pdf
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Biblioteca Central (FAUBA) de Universidad de Buenos Aires
Descripción
Sumario:This is the first report of the association between the rose-grain aphid Metopolophium dirhodum, a potentially important cereal pest and the facultative symbiont Hamiltonella defensa. The infection with this gamma-proteobacterium was determined by PCR in laboratory-reared and field-collected specimens of an Argentinian population of the aphid. Partial bacterial 16S, IGS and 23S rRNA genes were sequenced and compared to other available Hamiltonella sequences by phylogenetic analysis. The present study provides new information on previously unknown M. dirhodum microbiota.
ISSN:0325-7541

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