Transcriptional response of peripheral blood mononuclear cells from cattle infected with Mycobacterium bovis

Mostrar otras versiones (2)

Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and pro...

Descripción completa

Guardado en:
Detalles Bibliográficos
Otros Autores: Blanco, Federico Carlos, Soria, M. A., Bianco, María Verónica, Bigi, Fabiana
Formato: Artículo
Lenguaje:Inglés
Materias:
AMPHIREGULIN B
BIOLOGICAL MARKER
CALGRANULIN B
CD14 ANTIGEN
CHEMOKINE RECEPTOR CXCR2
CXCL9 CHEMOKINE
FC FRAGMENT IMMUNOGLOBULIN A RECEPTOR
GELATINASE B
HYALURONAN SYNTHASE 2
INTERLEUKIN 1 RECEPTOR
INTERLEUKIN 13 RECEPTOR ALPHA1
INTERLEUKIN 22
LOW DENSITY LIPOPROTEIN RECEPTOR RELATED PROTEIN
MANNOSE RECEPTOR C TYPE 1
MATRIX METALLOPROTEINASE 19
MONOCYTE CHEMOTACTIC PROTEIN 2
NEUROPILIN 1
RESISTIN
RNA
SH3 DOMAIN BINDING PROTEIN 4
THROMBOCYTE FACTOR 4
TRANSCRIPTION FACTOR
TRANSCRIPTION FACTOR FYVE
TRANSCRIPTION FACTOR GZMA
TRANSCRIPTION FACTOR THBS1
TRANSFORMING GROWTH FACTOR BETA
TRANSGLUTAMINASE 3
UNCLASSIFIED DRUG
UROKINASE RECEPTOR
VERY LOW DENSITY LIPOPROTEIN RECEPTOR
ADAM8 GENE
ADAMTSL4 GENE
ANIMAL CELL
ANIMAL EXPERIMENT
ANIMAL MODEL
ANIMAL TISSUE
APOPTOSIS
AREGB GENE
ARHGAP21 GENE
BOVINE TUBERCULOSIS
CATTLE DISEASE
CCL2 GENE
CCL8 GENE
CCR1 GENE
CD14 GENE
CELL TRANSPORT
CSF3R GENE
CXCL10 GENE
CXCL9 GENE
CXCR2 GENE
DEFBIO GENE
DOWN REGULATION
ECM1 GENE
ENDOCYTOSIS
FCAR GENE
FCGR1A GENE
FYVE GENE
GENE
GENE EXPRESSION PROFILING
GENETIC VARIABILITY
GZMA GENE
HAS2 GENE
IL 18 GENE
IL 1R GENE
IL 6 GENE
IL13RA1 GENE
IL1R1 GENE
IL1RN GENE
IL22 GENE
IMMUNE RESPONSE
INFLAMMATION
LOC508666 GENE
LOC533894 GENE
MAPK13 GENE
MAPK14 GENE
MICROARRAY ANALYSIS
MMP19 GENE
MMP3 GENE
MMP9 GENE
MRC1 GENE
MYCOBACTERIUM BOVIS
MYD88 GENE
NFAM1 GENE
NONHUMAN
NRP1 GENE
PERIPHERAL BLOOD MONONUCLEAR CELL
PF4 GENE
PLAUR GENE
PROTEIN DEGRADATION
PYCARD GENE
QUANTITATIVE ANALYSIS
RETN GENE
REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION
S100A9 GENE
SASH1 GENE
SH3BP4 GENE
TGFBI GENE
TGM3 GENE
THBS1 GENE
TREM1 GENE
TYROBP GENE
UPREGULATION
VLDLR GENE
ANIMALS
BIOLOGICAL MARKERS
CATTLE
GENE EXPRESSION REGULATION, BACTERIAL
LEUKOCYTES, MONONUCLEAR
MODELS, GENETIC
OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS
TRANSCRIPTION, GENETIC
TUBERCULOSIS, BOVINE
BOS
BOVINAE
Acceso en línea:http://ri.agro.uba.ar/files/download/articulo/2012Blanco.pdf
LINK AL EDITOR.
Aporte de:Registro referencial: Solicitar el recurso aquí
Biblioteca Central (FAUBA) de Universidad de Buenos Aires
Descripción
Sumario:Mycobacterium bovis is the causative agent of most cases of bovine tuberculosis. The identification of bTB biomarkers in specific stages of the disease will contribute to a better understanding of the immunopathology associated with tuberculosis and will enable their use in disease diagnosis and prognosis. The aim of this study was to evaluate the gene expression profile induced after specific stimulation of bovine peripheral blood mononuclear cells from cattle infected with M. bovis using the Affymetrix® GeneChip® Bovine Genome Array. A total of 172 genes showed differential expression profile that was statistically significant with log2-fold change grather than 2.5 and less than -2.5. Twenty-four out of these genes were upregulated and 148 were downregulated in bovine peripheral blood mononuclear cells of M. bovis-infected cattle. The highest differentially-expressed genes were related to immune and inflammatory responses, apoptosis, endocytosis, cellular trafficking and genes encoding proteins involved in cellular matrix degradation. Microarray results were confirmed in another group of infected cattle by RT-qPCR for the CD14, IL-1R, THBS1, MMP9 and FYVE genes. This study confirms previous findings that have shown that M. bovis infection in cattle results in the downregulation of immune response-related genes. Moreover, it validates the use of microarray platforms in combination with RT-qPCR to identify biomarkers of bovine tuberculosis. In addition, we propose CD14, IL-1R, THBS1, MMP9 and FYVE as potential biomarkers of bovine tuberculosis.
ISSN:1932-6203

Ejemplares similares