Heme oxygenase up - regulation under ultraviolet B radiation is not epigenetically restricted and involves specific stress-related transcriptions factors

Heme oxygenase-1 (HO-1) plays a protective role against oxidative stress in plants. The mechanisms regulating its expression, however, remain unclear. Here we studied the methylation state of a GC rich HO-1 promoter region and the expression of several stress-related transcription factors (TFs) in s...

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Detalles Bibliográficos
Otros Autores: Santa Cruz, Diego Mario, Pacienza, Natalia, Zilli, Carla G., Pagano, Eduardo Antonio, Balestrasse, Karina Beatriz, Yannarelli, Gustavo G.
Formato: Artículo
Lenguaje:Inglés
Materias:
HEME OXYGENASE
DNA METHYLATION
TRANSCRIPTION FACTORS
ULTRAVIOLET-B RADIATION
GLYCINE MAX
Acceso en línea:http://ri.agro.uba.ar/files/intranet/articulo/2017santacruz.pdf
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Biblioteca Central (FAUBA) de Universidad de Buenos Aires
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Sumario:Heme oxygenase-1 (HO-1) plays a protective role against oxidative stress in plants. The mechanisms regulating its expression, however, remain unclear. Here we studied the methylation state of a GC rich HO-1 promoter region and the expression of several stress-related transcription factors (TFs) in soybean plants subjected to ultraviolet-B (UV-B) radiation. Genomic DNA and total RNA were isolated from leaves of plants irradiated with 7.5 and 15 kJ m-2 UV-B. A 304 bp HO-1 promoter region was amplified by PCR from sodium bisulfite-treated DNA, cloned into pGEMT plasmid vector and evaluated by DNA sequencing. Bisulfite sequencing analysis showed similar HO-1 promoter methylation levels in control and UV-B-treated plants (C: 3.4±1.3%; 7.5: 2.6±0.5%; 15: 3.1±1.1%). Interestingly, HO-1 promoter was strongly unmethylated in control plants. Quantitative RT-PCR analysis of TFs showed that GmMYB177, GmMYBJ6, GmWRKY21, GmNAC11, GmNAC20 and GmGT2A but not GmWRK13 and GmDREB were induced by UV-B radiation. The expression of several TFs was also enhanced by hemin, a potent and specific HO inducer, inferring that they may mediate HO-1 upregulation. These results suggest that soybean HO-1 gene expression is not epigenetically regulated. Moreover, the low level of HO-1 promoter methylation suggests that this antioxidant enzyme can rapidly respond to environmental stress. Finally, this study has identified some stress-related TFs involved in HO-1 up-regulation under UV-B radiation.
ISSN:2213-2317

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