Culture of animal cells : a manual of basic technique /

Este libro es un recurso invaluable para los investigadores biomédicos en biología celular, citología, biología molecular, inmunología, neurociencia, toxicología y biología del cáncer.

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Detalles Bibliográficos
Autor principal: Freshney, R. Ian
Formato: Libro
Lenguaje:Español
Publicado: New York : Wiley-Liss, 1994
Edición:3rd. ed.
Materias:
Aporte de:Registro referencial: Solicitar el recurso aquí
Tabla de Contenidos:
  • Introduction
  • Biology of the Cultured Cell
  • Design and Layout of the Laboratory
  • Equipping the Laboratory
  • Aseptic Technique
  • Laboratory Safety and Biohazards
  • The culture environment: substrate, gas phase, medium and temperature
  • Preparation and sterilization
  • Disaggregation of the Tissue and primary Culture
  • Maintenance of the Culture: Cell Lines
  • Cloning and Selection of Specific Cell
  • Physical Methods of Cell Separation
  • Characterization
  • Induction of Differentiation
  • The Transformed Phenotype
  • Contamination
  • Instability, Variation and Preservation
  • Quantitation and Experimental Design
  • Measurement of viability and cytotoxicity
  • Culture of specific cell types
  • Culture of tumor tissue
  • Three-dimensional culture systems
  • Specialized techniques.