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Titulos:
Basic protein and peptide protocols / edited by John M. Walker.
ISBN:
089603268X; 0896032698 (comb)
Lugar de Edición:
Totowa, N.J. :
Editor:
Humana Press,
Fecha de Edición:
c1994.
Notas Formateada:
Lowry method for protein quantitation -- Bicinchoninic acid (BCA) assay for protein quantitation -- Bradford method for protein quantitation -- Nondenaturing polyacrylamide gel electrophoresis of proteins -- SDS polyacrylamide gel electrophoresis of proteins -- Gradient SDS polyacrylamide gel electrophoresis of proteins -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins -- Electrophoresis of peptides -- Isoelectric focusing of proteins in ultra-thin polyacrylamide gels -- In vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis -- Two-dimensional polyacrylamide gel electrophoresis of proteins -- Two-dimensional polyacrylamide gel electrophoresis using immobilized pH gradients in the first dimension.; (cont) Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension -- Quantification of proteins on polyacrylamide gels (nonradioactive) -- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique -- identification of glycoproteins on nitrocellulose membranes and gels -- Release of oligosaccharides from glycoproteins -- Structural profiling of oligosaccharides of glycoproteins -- Dying gels -- Fluorography of polyacrylamide gels containing tritium -- Electrophoretic elution of proteins from polyacrylamide gels -- Preparation of proteins from gels for protein microsequencing.; (cont) Comparison of protein primary structures: peptide mapping -- Protein blotting -- Detection of polypeptides on immunoblots using secondary antibodies or protein A -- Detection of proteins on protein blots using chemiluminescent systems -- Detection of proteins on blots using gold or immunogold -- Amino acid analysis -- Molecular-weight estimation for native proteins using size-exclusion high-performance liquid chromatography -- Peptide purification by reverse-phase HPLC -- Enzymatic methods for cleaving proteins -- Chemical cleavage of proteins -- Chemical modification of proteins -- Dansyl method for identifying N-terminal amino acids -- Dansyl-Edman method for peptide sequencing -- C-terminal sequencing of peptides: the thiocyanate degradation method.; (cont) Analysis of cysteine residues and disulfide bonds -- Preparation and characterization of monoclonal antibodies to proteins and other cellular components -- Raising of a polyclonal antiserum to a protein -- Production of antisera to synthetic peptides -- Production of antibodies using proteins in gel bands -- Preparation of purified immunoglobulin G (IgG) -- Enzyme and biotin labeling of antibody -- Labeling of peptides and proteins by radioiodination -- Radioimmunoassay of peptides and proteins -- Enzyme-linked immunosorbent assay (ELISA) -- Free zone capillary electrophoresis.
Palabras clave:
METODOS DE ANALISIS; Peptides; Peptides; PEPTIDOS; PROTEINAS; Proteins; Proteins

Leader:
cam
Campo 003:
OCoLC
Campo 008:
931215s1994 njua b 001 0 eng
Campo 020:
^a089603268X
Campo 020:
^a0896032698 (comb)
Campo 245:
00^aBasic protein and peptide protocols /^cedited by John M. Walker.
Campo 246:
Campo 260:
^aTotowa, N.J. :^bHumana Press,^cc1994.
Campo 300:
^axii, 490 p. :^bill. ;^c23 cm.
Campo 490:
1 ^aMethods in molecular biology ;^vv. 32
Campo 505:
0 ^aLowry method for protein quantitation -- Bicinchoninic acid (BCA) assay for protein quantitation -- Bradford method for protein quantitation -- Nondenaturing polyacrylamide gel electrophoresis of proteins -- SDS polyacrylamide gel electrophoresis of proteins -- Gradient SDS polyacrylamide gel electrophoresis of proteins -- Acetic acid-urea polyacrylamide gel electrophoresis of proteins -- Electrophoresis of peptides -- Isoelectric focusing of proteins in ultra-thin polyacrylamide gels -- In vivo isotopic labeling of proteins for polyacrylamide gel electrophoresis -- Two-dimensional polyacrylamide gel electrophoresis of proteins -- Two-dimensional polyacrylamide gel electrophoresis using immobilized pH gradients in the first dimension.
Campo 505:
0 ^a(cont) Two-dimensional polyacrylamide gel electrophoresis using flat-bed isoelectric focusing in the first dimension -- Quantification of proteins on polyacrylamide gels (nonradioactive) -- Detection of proteins in polyacrylamide gels using an ultrasensitive silver staining technique -- identification of glycoproteins on nitrocellulose membranes and gels -- Release of oligosaccharides from glycoproteins -- Structural profiling of oligosaccharides of glycoproteins -- Dying gels -- Fluorography of polyacrylamide gels containing tritium -- Electrophoretic elution of proteins from polyacrylamide gels -- Preparation of proteins from gels for protein microsequencing.
Campo 505:
0 ^a(cont) Comparison of protein primary structures: peptide mapping -- Protein blotting -- Detection of polypeptides on immunoblots using secondary antibodies or protein A -- Detection of proteins on protein blots using chemiluminescent systems -- Detection of proteins on blots using gold or immunogold -- Amino acid analysis -- Molecular-weight estimation for native proteins using size-exclusion high-performance liquid chromatography -- Peptide purification by reverse-phase HPLC -- Enzymatic methods for cleaving proteins -- Chemical cleavage of proteins -- Chemical modification of proteins -- Dansyl method for identifying N-terminal amino acids -- Dansyl-Edman method for peptide sequencing -- C-terminal sequencing of peptides: the thiocyanate degradation method.
Campo 505:
0 ^a(cont) Analysis of cysteine residues and disulfide bonds -- Preparation and characterization of monoclonal antibodies to proteins and other cellular components -- Raising of a polyclonal antiserum to a protein -- Production of antisera to synthetic peptides -- Production of antibodies using proteins in gel bands -- Preparation of purified immunoglobulin G (IgG) -- Enzyme and biotin labeling of antibody -- Labeling of peptides and proteins by radioiodination -- Radioimmunoassay of peptides and proteins -- Enzyme-linked immunosorbent assay (ELISA) -- Free zone capillary electrophoresis.
Campo 650:
4^aMETODOS DE ANALISIS
Campo 650:
22^aPeptides^xanalysis.
Campo 650:
0^aPeptides^xAnalysis^xMethodology.
Campo 650:
4^aPEPTIDOS
Campo 650:
4^aPROTEINAS
Campo 650:
12^aProteins^xanalysis.
Campo 650:
0^aProteins^xAnalysis^xMethodology.
Campo 700:
1 ^aWalker, John M.,^d1948-
Proveniencia:
^aUnCorQuim
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Institucion:
Base de Datos MARC21 - OCLC
Dependencia:
BDUMARC21

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